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After getting their Euro 2025 qualifying campaign off to the best possible start with a resounding victory over Belgium in Leuven, the Spanish national team face their second match on their way to the continental showpiece tournament. The Estadio El Plantío in Burgos is the venue for the national team's match against the Czech Republic. The World Champions have a new home kit for this match.
This is the official list of shirt numbers that will be worn by the Spanish National Football Team players called up by Montse Tomé to play the second match of the qualifying phase:
| SHIRT NUMBER | PLAYER NAME |
| 1 | Misa |
| 2 | O. Batlle |
| 3 | Teresa |
| 4 | Paredes |
| 5 | M. Méndez |
| 6 | Fiamma |
| 7 | Paralluelo |
| 8 | Mariona |
| 9 | Esther |
| 10 | J. Hermoso |
| 11 | Alexia |
| 12 | Vicky |
| 13 | A. Nanclares |
| 14 | Aleixandri |
| 15 | Eva Navarro |
| 16 | S. Lloris |
| 17 | L. García |
| 18 | M. Oroz |
| 19 | Olga |
| 20 | Shei |
| 21 | Redondo |
| 22 | Athenea |
| 23 | Lete |